99mTc-AgNPs-ICG: nanoparticle for hybrid image
DOI:
https://doi.org/10.35954/SM2024.43.1.6.e303Keywords:
silver compounds, 99mTc-HID, sodium pertechnetate Tc 99m, metal nanoparticles, indocyanine greenAbstract
Introduction: Currently nanotechnology has radically changed the diagnosis of many human pathologies. The aim of this work is to obtain silver nanoparticles for hybrid imaging (99mTc-AgNPs-ICG) having potential clinical imaging applications.
Materials and methods: We mixed 2 ml of ascorbic acid (1.7x10-4 M), 5 mCi of 99mTcO4-, 2 ml of citric acid (8.0 x 10-4 M) and 0.5 ml of silver nitrate (2.5 x 10-3 M). Solution pH was 5, and it was shaken for 20 minutes at 37º C. Afterwards, 2 µL of Indocyanine Green (1.3 x 10-3 M) was added (99mTc-AgNPs-ICG). Physiochemical properties of the solution were characterized by UV (λ1 = 420 nm, λ2 = 254 nm) and gamma detector. Fluorescence image, particle size and IR spectrum were evaluated.
Results: Silver nanoparticles were obtained in aqueous solution a pH of 5. Their pH, color and spectrum were stable for seven days. Furthermore, the principal peak characterized by HPLC, UV and Gamma detector had similar retention times. Its UV spectrum showed an absorption band of 420 nm, which corresponds to the plasmon absorption band of these nanoparticles. The particle size was 46 nm ± 1.5 nm. The IR spectrum showed absorption bands in 3193, 2624, 1596 y 1212 cm-1.
Conclusions: We describe for the first time in literature the synthesis of hybrid (radioactive and fluorescent) silver nanoparticles. Their physiochemical properties were characterized, being stable and their labelling was reproducible having potential biomedical applications.
Received for review: November 2023.
Accepted for publication: February 2024.
Correspondence: Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. Mataojo 2055, C.P. 11400, Montevideo, Uruguay. Tel: (+5982) 5250901/108; fax: (+5982) 5250895.
Contact e-mail: xcdamata@gmail.com
This article was approved by the Editorial Committee.
Downloads
References
Burdușel AC, Gherasim O, Grumezescu AM, Mogoantă L, Ficai A, et al. Applications of Silver Nanoparticles: An Up-to-Date Overview. Nanomaterials (Basel) 2018 Sep; 8(9):681. https://doi.org/10.3390/nano8090681
Clement JL, Jarrett PS. Antibacterial Silver. Met Based Drugs 1994; 1(5-6):467-482. https://doi.org/10.1155/MBD.1994.467
Saji VS, Choe HC, Young KWK. Nanotechnology in biomedical applications—a review. Int J Nano Biomater 2010; 3:119-139. https://doi.org/10.1504/IJNBM.2010.037801
Heiligtag FJ, Niederberger M. The fascinating world of nanoparticle research. Mater Today 2013; 16:262-271. https://doi.org/10.1016/j.mattod.2013.07.004
Syafiuddin A, Salmiati Salim MR, Kueh ABH, Hadibarata T, Nur H. A Review of silver nanoparticles: Research trends, global consumption, synthesis, properties, and future Challenges. J Clin Chem Soc 2017; 64:732-756. https://doi.org/10.1002/jccs.201700067
Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Approaches. Int J Mol Sci 2016 Sep 13; 17(9):piiE1534. https://doi.org/10.3390/ijms17091534
Rao CNR, Müller A, Cheetham AK. Nanomaterials – An Introduction. Cap. 1, p.1-11. https://doi.org/10.1002/352760247X.ch1
Sastry M. Moving Nanoparticles Around: Phase-Transfer Processes in Nanomaterials Synthesis. Cap. 3, p.31-50. In: Rao CNR, Müller A, Cheetham AK (Eds.). The Chemistry of Nanomaterials. Synthesis, Properties and ApplicationsWeinheim: Wiley-VCH Verlag GmbH & Co. KGaA, 2004. https://doi.org/10.1002/352760247X.ch3
Ouay BL, Stellacci F. Antibacterial activity of silver nanoparticles: A surface science insight. Nano Today 2015; 10:339-354. https://doi.org/10.1016/j.nantod.2015.04.002
Mie G. Beiträge zur Optik trüber Medien, speziell kolloidaler Metallösungen. Annalen der Physik 1908; 330(3):377-445. https://doi.org/10.1002/andp.19083300302
Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: Synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci 2016; 17:1534. https://doi.org/10.3390/ijms17091534
Ghosh R, Girigoswami K. NADH dehydrogenase subunits are overexpressed in cells exposed repeatedly to H2O2. Mutat Res 2008; 638(1-2):210-215. https://doi.org/10.1016/j.mrfmmm.2007.08.008
Asharani PV, Hande MP, Valiyaveettil S. Anti-proliferative activity of silver nanoparticles. BMC Cell Biol 2009; 10:65. Published 2009 Sep 17. https://doi.org/10.1186/1471-2121-10-65
Hsin YH, Chen CF, Huang S, Shih TS, Lai PS, Chueh PJ. The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells [published correction appears in Toxicol Lett 2008 Mar 10; 185(2):142]. Toxicol Lett 2008; 179(3):130-139. https://doi.org/10.1016/j.toxlet.2008.04.015
Sanpui P, Chattopadhyay A, Ghosh SS.Induction of apoptosis in cancer cells at low silver nanoparticle concentrations using chitosan nanocarrier. ACS Appl Mater Interfaces 2011; 3(2):218-228. https://doi.org/10.1021/am100840c
Ahamed M, Karns M, Goodson M, Rowe J, Hussain SM, Schlager JJ, et al. DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells. Toxicol Appl Pharmacol 2008; 233(3):404-410. https://doi.org/10.1016/j.taap.2008.09.015
Sukirtha R, Priyanka KM, Antony JJ,Kamalakkannan S, Thangam R, Gunasekaran P, et al. Cytotoxic effect of green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model. Process Biochem 2012; 47:273-279. https://doi.org/10.1016/j.procbio.2011.11.003
Chen D, Dougherty CA, Yang D, Wu H, Hong H. Radioactive Nanomaterials for Multimodality Imaging. Tomography 2016 Mar; 2(1):3-16. https://doi.org/10.18383/j.tom.2016.00121
Gambini JP, Silvera E, Musetti M, Quinn T, Zhong Yang G, Matalonga S, et al. 99mTc nanocolloid indicyanine green: An hybrid tracer for breast sentinel node procedures. J Nucl Med 2019 May 1; (60)supplement 1:1231.
Ider M, Abderrafi K, Eddahbi A, Ouaskit S, Kassiba A. Silver Metallic Nanoparticles with Surface Plasmon Resonance: Synthesis and Characterizations. J Clust Sci 2017; 28:1051-1069. https://doi.org/10.1007/s10876-016-1080-1
Herrmann K, Nieweg OE, Povoski SP (Eds.). Radioguided surgery. Current Applications and Innovative Directions in Clinical Practice. Switzerland: Springer International, 2016.
Liu P, Huang Z, Chen Z, Xu R, Wu H, Zang F, et al. Silver nanoparticles: a novel radiation sensitizer for glioma? Nanoscale 2013; 5(23):11829-11836. https://doi.org/10.1039/c3nr01351k
Liu Z, Tan H, Zhang X, Zhou Z, Hu X, Zhang H, et al. Enhancement of radiotherapy efficacy by silver nanoparticles in hypoxic glioma cells. Artif Cells Nanomed Biotechnol 2018; 46(sup3):S922-S930. https://doi.org/10.1080/21691401.2018.1518912
Zhao J, Li D, Ma J, Yang H, Chen W, Cao Y, et al. Increasing the accumulation of aptamer AS1411 and verapamil conjugated silver nanoparticles in tumor cells to enhance the radiosensitivity of glioma. Nanotechnology 2021; 32(14):145102. https://doi.org/10.1088/1361-6528/abd20a
Lee SH, Jun BH. Silver Nanoparticles: Synthesis and Application for Nanomedicine. Int J Mol Sci 2019 Feb 17; 20(4):865. https://doi.org/10.3390/ijms20040865
Marimuthu S, Rahuman AA, Rajakumar G, Santhoshkumar T, Kirthi AV, Jayaseelan C, et al. Evaluation of green synthesized silver nanoparticles against parasites. Parasitology Research 2011; 108(6):1541-1549. https://doi.org/10.1007/s00436-010-2212-4
Frost MS, Dempsey MJ, Whitehead DE. The response of citrate functionalised gold and silver nanoparticles to the addition of heavy metal ions. Colloids and Surfaces A: Physicochemical and Engineering Aspects 2017 Apr 5; 518:15-24. https://doi.org/10.1016/j.colsurfa.2016.12.036
Sheng Z, Hu D, Xue M, He M, Gong P, Cai L. Indocyanine Green Nanoparticles for Theranostic Applications. Nano-Micro Lett 2013; 5:145-150. https://doi.org/10.1007/BF03353743
Ding J, Chen G, Chen G, Guo M. One-Pot Synthesis of Epirubicin-Capped Silver Nanoparticles and Their Anticancer Activity against Hep G2 Cells. Pharmaceutics 2019 Mar 15; 11(3):123. https://doi.org/10.3390/pharmaceutics11030123
De Matteis V, Cascione M, Toma CC, Leporatti S. Silver Nanoparticles: Synthetic Routes, In Vitro Toxicity and Theranostic Applications for Cancer Disease. Nanomaterials (Basel) 2018 May 10; 8(5):319. https://doi.org/10.3390/nano8050319
Gonzalez AL, Noguezn C, Beranek J, Barnard AS. Size, Shape, Stability, and Color of Plasmonic Silver Nanoparticles. J Phys Chem C 2014; 118:9128-9136. https://doi.org/10.1021/jp5018168
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 : Stephanie Simois, Agostina Cammarata, Romina Glisoni, Mirel Cabrera, Marcos Tassano, Juan Pablo Gambini, Ximena Aida Camacho Damata, Pablo Cabral. The author retains his copyright and assigns to the journal the right of first publication of his work, which will be simultaneously subject to the Creative Commons Attribution 4.0 International License that allows sharing the work as long as the initial publication in this journal is indicated.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Until 2024 we use the Creative Commons Attribution/NonCommercial Attribution 4.0 International License https://creativecommons.org/licenses/by-nc/4.0/deed.es. Which states that: you are free to share, copy and redistribute the material in any medium or format, as well as to adapt, remix, transform and build upon the material. Under the following terms:
Attribution: you must give proper credit , provide a link to the license, and indicate if changes have been made . You may do so in any reasonable manner, but not in such a way as to suggest that you or your use is endorsed by the licensor.
NonCommercial: you may not use the material for commercial purposes.
As of 2025 authors retain their copyright and assign to the journal the right of first publication of their work, which shall simultaneously be subject to the license https://creativecommons.org/licenses/by-nc-sa/4.0/deed.es that permits sharing, copying and redistribution of the material in any medium or format provided that initial publication in this journal is indicated. Adapt, remix, transform and build upon the material. If you remix, transform, or build from the material, you must distribute your contribution under the same license as the original and may not make use of the material for commercial purposes.
Under the following terms:
1. Attribution: you must give proper credit, provide a link to the license, and indicate whether changes have been made. You may do so in any reasonable manner, but not in such a way as to suggest that you or your use is endorsed by the licensor.
2. NonCommercial: you may not use the material for commercial purposes.
3. ShareAlike: if you remix, transform or build upon the material, you must distribute your contribution under the same license as the original.
PlumX Metrics
